首页|Vacuolar proteomic analysis reveals tonoplast transporters for accumulation of citric acid and sugar in citrus fruit
Vacuolar proteomic analysis reveals tonoplast transporters for accumulation of citric acid and sugar in citrus fruit
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Vacuole largely dictates the fruit taste and flavor,as most of the sugars and organic acids are stored in the vacuoles of the fruit.However,difficulties associated with vacuole separation severely hinder identification and characterization of vacuolar proteins in fruit species.In this study,we established an effective approach for separating vacuoles and successfully purified vacuolar protein from six types of citrus fruit with varying patterns of sugar and organic acid contents.By using label-free LC-MS/MS proteomic analysis,1443 core proteins were found to be associated with the essential functions of vacuole in citrus fruit.Correlation analysis of metabolite concentration with proteomic data revealed a transporter system for the accumulation of organic acid and soluble sugars in citrus.Furthermore,we characterized the physiological roles of selected key tonoplast transporters,ABCG15,Dict2.1,TMT2,and STP7 in the accumulation of citric acid and sugars.These findings provide a novel perspective and practical solution for investigating the transporters underlying the formation of citrus taste and flavor.
Zuolin Mao、Yue Wang、Mengdi Li、Shuhang Zhang、Zeqi Zhao、Qiang Xu、Ji-Hong Liu、Chunlong Li
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National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China
Hubei Hongshan Laboratory,Wuhan 430070,China
国家重点研发计划国家自然科学基金Foundation of Hubei Hongshan Laboratory中央高校基本科研业务费专项Huazhong Agricultural University(startup funding)
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