首页|A telomere-to-telomere reference genome of ficus(Ficus hispida)provides new insights into sex determination

A telomere-to-telomere reference genome of ficus(Ficus hispida)provides new insights into sex determination

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A high-quality reference genome is indispensable for resolving biologically essential traits.Ficus hispida is a dioecious plant.A complete Ficus reference genome will be crucial for understanding their sex evolution and important biological characteristics,such as aerial roots,mutualistic symbiosis with ficus-wasps,and fruiting from old stems.Here,we generated a telomere-to-telomere(T2T)genome for F.hispida using PacBio HiFi and Oxford Nanopore Ultra-long sequencing technologies.The genome contiguity and completeness has shown improvement compared with the previously released genome,with the annotation of six centromeres and 28 telomeres.We have refined our previously reported 2-Mb male-specific region into a 7.2-Mb genomic region containing 51 newly predicted genes and candidate sex-determination genes AG2 and AG3.Many of these genes showed extremely low expression,likely attributed to hypermethylation in the gene body and promoter regions.Gene regulatory networks(GRNs)revealed that AG2 and AG3 are related to the regulation of stamen development in male flowers,while the AG1 gene is responsible for regulating female flowers'defense responses and secondary metabolite processes.Comparative analysis of GRNs showed that the NAC,WRKY,and MYB transcription factor families dominate the female GRN,whereas the MADS and MYB transcription factor families are prevalent in the male GRN.

Zhenyang Liao、Tianwen Zhang、Wenlong Lei、Yibin Wang、Jiaxin Yu、Yinghao Wang、Kun Chai、Gang Wang、Huahao Zhang、Xingtan Zhang

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Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong 518120,China

CAS Key Laboratory of Tropical Forest Ecology,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Mengla,Yunnan 666303,China

College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou 350002,China

College of Pharmacy and Life Science,Jiujiang University,Jiujiang 332005,China

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国家自然科学基金Key Programs of Jiangxi Youth Science Foundation

3220018820202ACBL215008

2024

10.1093/hr/uhad257

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(1)
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