首页|A Ca2+sensor BraCBL1.2 involves in BraCRa-mediated clubroot resistance in Chinese cabbage

A Ca2+sensor BraCBL1.2 involves in BraCRa-mediated clubroot resistance in Chinese cabbage

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Clubroot disease caused by Plasmodiophora brassicae(P.brassicae)severely threatens the cultivation of Cruciferous plants,especially Chinese cabbage.Recently,resistance genes in plants have been reported to encode for a Ca2+-permeable channel in the plasma membrane,which can mediate the cytosolic Ca2+increase in plant cells upon pathogen attack.However,the downstream Ca2+sensor and decoder are still unknown.In this study,we identified the virulent and avirulent P.brassicae isolates(Pbs)of two near isogenic lines,CR 3-2 and CS 3-2,with CR 3-2 harboring clubroot resistant gene BraCRa.The transcriptomic analysis was then conducted with CR 3-2 after inoculating with virulent isolate PbE and avirulent isolate Pb4.From the differentially expressed genes of transcriptomic data,we identified a Ca2+-sensor encoding gene,BraCBL1.2,that was highly induced in CR 3-2 during infection by Pb4 but not by PbE.Moreover,GUS histochemical staining and subcellular localization analysis revealed that BraCBL1.2 was specifically expressed in the root hair cells of Arabidopsis and encoded a putative Ca2+sensor localized in the plasma membrane.We also developed an assay to investigate the BraCRa-mediated hypersensitive response(HR)in tobacco leaves.The results suggest that BraCBL1.2 is involved in the BraCRa-mediated plant ETI immune response against P.brassicae.In addition,we verified that overexpression of BraCBL1.2 enhanced clubroot resistance in Arabidopsis.Collectively,our data identified the involvement of a Ca2+sensor in BraCRa-mediated clubroot resistance in Chinese cabbage,providing a theoretical basis for further research on the resistance of Chinese cabbage to P.brassicae.

Yinglan Piao、Shizhen Li、Yiduo Chen、Sisi Zhao、Zhongyun Piao、Haiping Wang

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State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China

State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China

Institut für Biologie und Biotechnologie der Pflanzen,Westfälische Wilhelms-Universität,Münster 48143,Germany

College of Horticulture,Shenyang Agricultural University,Shenyang 110866,China

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国家重点研发计划China Scholarship Council,National Modern Agricultural Industry Technology System Construction Special Fund Project国家自然科学基金Innovation Engineering Project of Chinese Academy of Agricultural SciencesMinistry of Agriculture and Rural Affairs Crop Germplasm Resources Safety Conservation ProjectNational Horticultural Germplasm Beijing Vegetable Subbank Operation Service ProjectObservation and Monitoring Mission Project of National Crop Germplasm Resources Data Center

2021YFD1200200CARS-24-A-0131872946CAAS-ASTIP-2021-IVFCAAS2022NWB037NHGRC2022-NH01ZX01NK-03

2024

10.1093/hr/uhad261

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(1)
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