首页|A QTL of eggplant shapes the rhizosphere bacterial community,co-responsible for resistance to bacterial wilt

A QTL of eggplant shapes the rhizosphere bacterial community,co-responsible for resistance to bacterial wilt

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Resistant crop cultivars can recruit beneficial rhizobacteria to resist disease.However,whether this recruitment is regulated by quantitative trait loci(QTL)is unclear.The role of QTL in recruiting specific bacteria against bacterial wilt(BW)is an important question of practical significance to disease management.Here,to identify QTL controlling BW resistance,Super-BSA was performed in F2 plants derived from resistant eggplant cultivar R06112 × susceptible cultivar S55193.The QTL was narrowed down through BC1F1-BC3F1 individuals by wilting symptoms and KASP markers.Rhizosphere bacterial composition of R06112,S55193,and resistant individuals EB158(with the QTL)and susceptible individuals EB327(without QTL)from BC2F1 generation were assessed by Illumina sequencing-based analysis,and the activation of plant immunity by the bacterial isolates was analyzed.Evidence showed that BW-resistant is controlled by one QTL located at the 270 kb region on chromosome 10,namely EBWR10,and nsLTPs as candidate genes confirmed by RNA-Seq.EBWR10 has a significant effect on rhizobacteria composition and significantly recruits Bacillus.pp.A SynCom of three isolated Bacillus.pp trains significantly reduced the disease incidence,changed activities of CAT,PPO,and PAL and concentration of NO,H2O2,and O2-,activated SA and JA signaling-dependent ISR,and displayed immune activation against Ralstonia solanacearum in eggplant.Our findings demonstrate for the first time that the QTL can recruit beneficial rhizobacteria,which jointly promote the suppression of BW.This method charts a path to develop the QTL in resistant cultivar-driven probiotics to ameliorate plant diseases.

Chao Gong、Zhenshuo Wang、Zhiliang Li、Baojuan Sun、Wenlong Luo、Shanwei Luo、Shuting Chen、Peiting Mai、Zhenxing Li、Ye Li、Yikui Wang、Tao Li

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Guangdong Academy of Agricultural Sciences,Guangdong Key Laboratory for New Technology Research of Vegetables,Vegetable Research Institute,Guangzhou,510640,China

Department of Plant Pathology,MOA Key Lab of Pest Monitoring and Green Management,College of Plant Protection,China Agricultural University,Beijing 100193,China

Harbin Academy of Agricultural Sciences,Harbin,Heilongjiang,150029,China

Institute of Vegetable,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi,530007,China

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major special projects of the Guangxi Science and Technology ProgramGuangdong Basic and Applied Basic Research Foundation国家自然科学基金Guangzhou Basic and Applied Basic Research FoundationDepartment of Agriculture and Rural Areas of Guangdong Province of ChinaDepartment of Agriculture and Rural Areas of Guangdong Province of ChinaDepartment of Agriculture and Rural Areas of Guangdong Province of ChinaDepartment of Agriculture and Rural Areas of Guangdong Province of ChinaSpecial Fund for Scientific Innovation Strategy-Construction of High Leveled Academy of Agriculture ScienceSpecial Fund for Scientific Innovation Strategy-Construction of High Leveled Academy of Agriculture ScienceSpecial Fund for Scientific Innovation Strategy-Construction of High Leveled Academy of Agriculture ScienceScientific and technological research project of Harbin Science and Technology Department

AA22068088-22021A1515012490323727022022010102472023KJ1102023KJ1062022-NJS-00-0052022-NPY-00-026202114TDR2019PY-JX003R2023PY-JX0082021ZSZZNS07

2024

10.1093/hr/uhad272

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(2)
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