首页|MicroRNA396 negatively regulates shoot regeneration in tomato

MicroRNA396 negatively regulates shoot regeneration in tomato

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Numerous studies have been dedicated to genetically engineering crops to enhance their yield and quality.One of the key requirements for generating genetically modified plants is the reprogramming of cell fate.However,the efficiency of shoot regeneration during this process is highly dependent on genotypes,and the underlying molecular mechanisms remain poorly understood.Here,we identified microRNA396(miR396)as a negative regulator of shoot regeneration in tomato.By selecting two genotypes with contrasting shoot regeneration efficiencies and analyzing their transcriptome profiles,we found that miR396 and its target transcripts,which encode GROWTH-REGULATING FACTORs(GRFs),exhibit differential abundance between high-and low-efficiency genotypes.Suppression of miR396 functions significantly improved shoot regeneration rates along with increased expression of GRFs in transformed T0 explants,suggesting that miR396 is a key molecule involved in the determination of regeneration efficiency.Notably,we also showed that co-expression of a miR396 suppressor with the gene-editing tool can be employed to generate gene-edited plants in the genotype with a low capacity for shoot regeneration.Our findings show the critical role of miR396 as a molecular barrier to shoot regeneration in tomato and suggest that regeneration efficiency can be improved by blocking this single microRNA.

Su-Jin Park、Ji-Sun Park、Jin Ho Yang、Ki-Beom Moon、Seung Yong Shin、Jae-Heung Jeon、Hyun-Soon Kim、Hyo-Jun Lee

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Plant Systems Engineering Research Center,Korea Research Institute of Bioscience and Biotechnology,125 Gwahak-ro,Daejeon 34141,Korea

Department of Biosystems and Bioengineering,KRIBB School of Biotechnology,University of Science and Technology,125 Gwahak-ro,Daejeon 34113,Korea

Department of Functional Genomics,KRIBB School of Bioscience,University of Science and Technology,125 Gwahak-ro,Daejeon 34113,Korea

Department of Biological Sciences,Sungkyunkwan University,2066 Seobu-ro,Suwon 16419,Korea

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New Breeding Technologies Development ProgramRural Development Administration of KoreaBasic Research Program provided by the National Research Foundation of KoreaBasic Research Program provided by the National Research Foundation of KoreaKRIBB Research Initiative ProgramsKRIBB Research Initiative Programs

PJ01653001NRF-2023R1A2C1003963NRF-2022R1I1A2073565KGM5372322KGM1002311

2024

10.1093/hr/uhad291

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(2)
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