首页|Cuticular wax biosynthesis in blueberries(Vaccinium corymbosum L.):Transcript and metabolite changes during ripening and storage affect key fruit quality traits

Cuticular wax biosynthesis in blueberries(Vaccinium corymbosum L.):Transcript and metabolite changes during ripening and storage affect key fruit quality traits

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In fruits,cuticular waxes affect fruit quality traits such as surface color at harvest and water loss during postharvest storage.This study investigated the transcriptional regulation of cuticular wax deposition in northern highbush blueberries(Vaccinium corymbosum L.)in relation to fruit water loss and surface color during ripening and postharvest storage,as well as the effects of abscisic acid(ABA)-mediated changes in cuticular wax deposition on these fruit quality traits.Total cuticular wax content(μg·cm-2)decreased during fruit ripening and increased during postharvest storage.Transcriptome analysis revealed a transcript network for cuticular wax deposition in blueberries.Particularly,five OSC-Likes were identified as putative genes for triterpene alcohol production,with OSC-Like1 and OSC-Like2 encoding mixed amyrin synthases,OSC-Like3 encoding a lupeol synthase,and OSC-Like4 and OSC-Like5 encoding cycloartenol synthases.The expression of three CYP716A-like genes correlated to the accumulation of two triterpene acids oleanolic acid and ursolic acid,the major wax compounds in blueberries.Exogenous ABA application induced the expression of triterpenoid biosynthetic genes and the accumulation of β-amyrin and oleanolic acid,as well as increased the ratio of oleanolic acid to ursolic acid.These changes were associated with reduced fruit water loss.The content of β-diketones was also increased by ABA application,and this increase was associated with increased fruit lightness(measured as L*using CIELAB Color Space by a colorimeter).This study provided key insights on the molecular basis of cuticular wax deposition and its implications on fruit quality traits in blueberries.

Triterpenoidsβ-diketonestranscriptomicsabscisic acidfruit water lossfruit color

Yifan Yan、Kristina K.Gagalova、Eric M.Gerbrandt、Simone D.Castellarin

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Wine Research Centre,Faculty of Land and Food Systems,The University of British Columbia,2205 East Mall,Vancouver,BC V6T 1Z4,Canada

Canada's Michael Smith Genome Sciences Centre,570 W 7th Ave,Vancouver,BC V5Z 4S6,Canada

British Columbia Blueberry Council,32160 South Fraser Way #275,Abbotsford,BC V2T 1W5,Canada

Canadian Agricultural Partnership,a federalprovincialterritorial initiativeCanada-BC Agri-Innovation ProgramInvestment Agriculture Foundation of BCBritish Columbia Blueberry Council(BCBC)MITACS Program

INV098IT14908

2024

10.1093/hr/uhae004

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(3)
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