首页|Genome and GWAS analysis identified genes significantly related to phenotypic state of Rhododendron bark

Genome and GWAS analysis identified genes significantly related to phenotypic state of Rhododendron bark

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As an important horticultural plant,Rhododendron is often used in urban greening and landscape design.However,factors such as the high rate of genetic recombination,frequent outcrossing in the wild,weak linkage disequilibrium,and the susceptibility of gene expression to environmental factors limit further exploration of functional genes related to important horticultural traits,and make the breeding of new varieties require a longer time.Therefore,we choose bark as the target trait which is not easily affected by environmental factors,but also has ornamental properties.Genome-wide association study(GWAS)of Rhododendron delavayi(30 samples),R.irroratum(30 samples)and their F1 generation R.agastum(200 samples)was conducted on the roughness of bark phenotypes.Finally,we obtained 2416.31 Gbp of clean data and identified 5 328800 high-quality SNPs.According to the P-value and the degree of linkage disequilibrium of SNPs,we further identified 4 out of 11 candidate genes that affect bark roughness.The results of gene differential expression analysis further indicated that the expression levels of Rhdel02G0243600 and Rhdel08G0220700 in different bark phenotypes were significantly different.Our study identified functional genes that influence important horticultural traits of Rhododendron,and illustrated the powerful utility and great potential of GWAS in understanding and exploiting wild germplasm genetic resources of Rhododendron.

Qiannan Ye、Lu Zhang、Qing Li、Yaliang Ji、Yanli Zhou、Zhenzhen Wu、Yanting Hu、Yongpeng Ma、Jihua Wang、Chengjun Zhang

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Germplasm Bank of Wild Species,Yunnan Key Laboratory for Crop Wild Relatives Omics,Kunming Institute of Botany,Chinese Academy of Science,Kunming,Yunnan 650201,China

University of Chinese Academy of Sciences,Beijing 100049,China

Flower Research Institute of Yunnan Academy of Agricultural Sciences,National Engineering Research Center for Ornamental Horticulture,Yunnan Academy of Agricultural Sciences Kunming 650000,China

State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China

Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China

Haiyan Engineering & Technology Center,Zhejiang Institute of Advanced Technology,Jiaxing 314022,China

State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,China

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ZIAT-Special ProjectScience and Technology Talent and Platform Program of Yunnan ProvinceYunnan Province Agriculture joint special projectChina Agriculture Research System of MOF and MARA

ZJKJT-2022-01202105 AC160017202101BD070001-010CARS-23-G56

2024

10.1093/hr/uhae008

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(3)
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