首页|A haplotype-resolved gap-free genome assembly provides novel insight into monoterpenoid diversification in Mentha suaveolens'Variegata'

A haplotype-resolved gap-free genome assembly provides novel insight into monoterpenoid diversification in Mentha suaveolens'Variegata'

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Mentha is a commonly used spice worldwide,which possesses medicinal properties and fragrance.These characteristics are conferred,at least partially,by essential oils such as menthol.In this study,a gap-free assembly with a genome size of 414.3 Mb and 31,251 coding genes was obtained for Mentha suaveolens'Variegata'.Based on its high heterozygosity(1.5％),two complete haplotypic assemblies were resolved,with genome sizes of 401.9 and 405.7 Mb,respectively.The telomeres and centromeres of each haplotype were almost fully annotated.In addition,we detected a total of 41,135 structural variations.Enrichment analysis demonstrated that genes involved in terpenoid biosynthesis were affected by these structural variations.Analysis of volatile metabolites showed that M.suaveolens mainly produces piperitenone oxide rather than menthol.We identified three genes in the M.suaveolens genome which encode isopiperitenone reductase(ISPR),a key rate-limiting enzyme in menthol biosynthesis.However,the transcription levels of ISPR were low.Given that other terpenoid biosynthesis genes were expressed,M.suaveolens ISPRs may account for the accumulation of piperitenone oxide in this species.The findings of this study may provide a valuable resource for improving the detection rate and accuracy of genetic variants,thereby enhancing our understanding of their impact on gene function and expression.Moreover,our haplotype-resolved gap-free genome assembly offers novel insights into molecular marker-assisted breeding of Mentha.

Hanting Yang、Can Wang、Guanru Zhou、Yuxuan Zhang、Tianxing He、Lulu Yang、Ya Wu、Zhengnan Wang、Xin Tang、Gang Chen、Zhaoyu Liu、Huanyu Tang、Hanlin Zhou、Xumei Kang、Sanyin Zhang、Liang Leng、Shilin Chen、Chi Song

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Institute of Herbgenomics,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China

Pharmacy College,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China

Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China

Hubei University of Chinese Medicine,Wuhan 430065,China

Wuhan Benagen Technology Co.,Ltd,Wuhan 430000,China

Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China

Chongqing Academy of Chinese Materia Medica,Chongqing College of Traditional Chinese Medicine,Chongqing,China

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introduces the talented person scientific research start funds subsidization project of Chengdu University of Traditional Chinesintroduces the talented person scientific research start funds subsidization project of Chengdu University of Traditional ChinesHubei science and technology planning project

0300400150300400172020BCB038

2024

10.1093/hr/uhae022

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(3)
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