首页|Two large inversions seriously suppress recombination and are essential for key genotype fixation in cabbage(Brassica oleracea L.var.capitata)

Two large inversions seriously suppress recombination and are essential for key genotype fixation in cabbage(Brassica oleracea L.var.capitata)

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
Chromosomal inversion is an important structural variation that usually suppresses recombination and is critical for key genotype fixation.In a previous study,an 11.47 Mb recombination suppression region was identified in the yellow-green leaf locus BoYgl-1 on chromosome 1,but the cause of recombination suppression is still unclear.In this study,chlorophyll and carotenoid contents were found to be significantly decreased in the yellow-green leaf mutant YL-1.Genome assembly and comparative analysis revealed that two large inversions in YL-1 were responsible for the severe recombination suppression in the BoYgl-1 locus.Analyses with inversion-specific markers revealed that the inversions were present in 44(including all wild cabbage;INV1 and INV2)of 195 cabbage inbred lines and 15(INV1)ornamental kale inbred lines,indicating that these species with INV1 or INV2 may have evolved much earlier than other types of cabbage.Analyses with inversion-correlated markers revealed that the genotypes of CoINV1,CoINV2 and CoINV3 were highly correlated with INV1 and INV2,indicating that INVs could fix the key genotypes of the involved region.In addition,a 5.87 Mb assembly inversion was identified at the BoYgl-1 locus in the TO1000 genome by genome comparative analysis.This study provides new insight into the recombination suppression mechanism of chromosomal inversion and the application of genome fragment fixation in cabbage breeding.

Bin Zhang、Yuankang Wu、Shoufan Li、Limei Yang、Mu Zhuang、Honghao Lv、Yong Wang、Jialei Ji、Xilin Hou、Fengqing Han、Yangyong Zhang

展开 >

State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China

State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China

国家自然科学基金

31872948

2024

10.1093/hr/uhae030

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(4)
  • 24