首页|Characterization of O-methyltransferases in the biosynthesis of phenylphenalenone phytoalexins based on the telomere-to-telomere gapless genome of Musella lasiocarpa

Characterization of O-methyltransferases in the biosynthesis of phenylphenalenone phytoalexins based on the telomere-to-telomere gapless genome of Musella lasiocarpa

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Phenylphenalenones(PhPNs),phytoalexins in wild bananas(Musaceae),are known to act against various pathogens.However,the abundance of PhPNs in many Musaceae plants of economic importance is low.Knowledge of the biosynthesis of PhPNs and the application of biosynthetic approaches to improve their yield is vital for fighting banana diseases.However,the processes of PhPN biosynthesis,especially those involved in methylation modification,remain unclear.Musella lasiocarpa is a herbaceous plant belonging to Musaceae,and due to the abundant PhPNs,their biosynthesis in M.lasiocarpa has been the subject of much attention.In this study,we assembled a telomere-to-telomere gapless genome of M.lasiocarpa as the reference,and further integrated transcriptomic and metabolomic data to mine the candidate genes involved in PhPN biosynthesis.To elucidate the diversity of PhPNs in M.lasiocarpa,three screened O-methyltransferases(Ml01G0494,Ml04G2958,and Ml08G0855)by phylogenetic and expressional clues were subjected to in vitro enzymatic assays.The results show that the three were all novel O-methyltransferases involved in the biosynthesis of PhPN phytoalexins,among which Ml08G0855 was proved to function as a multifunctional enzyme targeting multiple hydroxyl groups in PhPN structure.Moreover,we tested the antifungal activity of PhPNs against Fusarium oxysporum and found that the methylated modification of PhPNs enhanced their antifungal activity.These findings provide valuable genetic resources in banana breeding and lay a foundation for improving disease resistance through molecular breeding.

Wanli Zhao、Junzhi Wu、Mei Tian、Shu Xu、Shuaiya Hu、Zhiyan Wei、Guyin Lin、Liang Tang、Ruiyang Wang、Boya Feng、Bi Wang、Hui Lyu、Christian Paetz、Xu Feng、Jia-Yu Xue、Pirui Li、Yu Chen

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Jiangsu Key Laboratory for the Research and Utilization of Plant Resources,Jiangsu Province Engineering Research Center of Eco-cultivation and High-value Utilization of Chinese Medicinal Materials,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem.Sun Yat-Sen),210014 Nanjing,China

Nanjing University of Chinese Medicine,210023 Nanjing,China

College of Horticulture,Bioinformatics Center,Academy for Advanced Interdisciplinary Studies,Nanjing Agricultural University,210095 Nanjing,China

NMR/Biosynthesis Group,Max Planck Institute for Chemical Ecology,Hans-Knöll-Straße 8,07745 Jena,Germany

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国家自然科学基金国家自然科学基金国家自然科学基金江苏省自然科学基金江苏省自然科学基金Jiangsu Institute of Botany Talent Fund

320703603220032622207047BK20220752BK20220749JIBTF202304

2024

10.1093/hr/uhae042

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(4)
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