首页|Engineered dsRNA-protein nanoparticles for effective systemic gene silencing in plants

Engineered dsRNA-protein nanoparticles for effective systemic gene silencing in plants

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Long-distance transport or systemic silencing effects of exogenous biologically active RNA molecules in higher plants have not been reported.Here,we report that cationized bovine serum albumin(cBSA)avidly binds double-stranded beta-glucuronidase RNA(dsGUS RNA)to form nucleic acid-protein nanocomplexes.In our experiments with tobacco and poplar plants,we have successfully demonstrated systemic gene silencing effects of cBSA/dsGUS RNA nanocomplexes when we locally applied the nanocomplexes from the basal ends of leaf petioles or shoots.We have further demonstrated that the cBSA/dsGUS RNA nanocomplexes are highly effective in silencing both the conditionally inducible DR5-GUS gene and the constitutively active 35S-GUS gene in leaf,shoot,and shoot meristem tissues.This cBSA/dsRNA delivery technology may provide a convenient,fast,and inexpensive tool for characterizing gene functions in plants and potentially for in planta gene editing.

Huayu Sun、Ankarao Kalluri、Dan Tang、Jingwen Ding、Longmei Zhai、Xianbin Gu、Yanjun Li、Huseyin Yer、Xiaohan Yang、Gerald A.Tuskan、Zhanao Deng、Frederick G.Gmitter Jr、Hui Duan、Challa Kumar、Yi Li

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Department of Plant Science and Landscape Architecture,University of Connecticut,Storrs,CT 06269,USA

Institute of Gene Science and Industrialization for Bamboo and Rattan Resources,International Center for Bamboo and Rattan,Beijing 100102,China

Department of Material Science,University of Connecticut,Storrs,CT 06269,USA

Department of Chemistry,University of Connecticut,Storrs,CT 06269,USA

Biosciences Division,Center for Bioenergy Innovation,Oak Ridge National Laboratory,Oak Ridge,TN 37831,USA

Gulf Coast Research and Education Center,University of Florida,IFAS,Wimauma,FL 33598,USA

Citrus Research and Education Center,University of Florida,IFAS,Lake Alfred,FL 33850,USA

USDA-ARS,U.S.National Arboretum,Floral and Nursery Plants Research Unit,Beltsville Agricultural Research Center(BARC)-West,Beltsville,MD 20705,USA

Department of Molecular and Cellular Biology,University of Connecticut,Storrs,CT 06269,USA

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USDA National Institute of Food and Agriculture SCRIConnecticut-Storrs Agriculture Experimental Station

2015-70016-23027

2024

10.1093/hr/uhae045

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(4)
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