首页|Banana MabHLH28 positively regulates the expression of softening-related genes to mediate fruit ripening independently or via cooperating with MaWRKY49/111
Banana MabHLH28 positively regulates the expression of softening-related genes to mediate fruit ripening independently or via cooperating with MaWRKY49/111
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Texture softening is a physiological indicator of fruit ripening,which eventually contributes to fruit quality and the consumer's accep-tance.Despite great progress having been made in identification of the genes related to fruit softening,the upstream transcriptional regulatory pathways of these softening-related genes are not fully elucidated.Here,a novel bHLH gene,designated as MabHLH28,was identified because of its significant upregulation in banana fruit ripening.DAP-Seq analysis revealed that MabHLH28 bound to the core sequence of'CAYGTG'presented in promoter regions of fruit softening-associated genes,such as the genes related to cell wall modification(MaPG3,MaPE1,MaPL5,MaPL8,MaEXP1,MaEXP2,MaEXPA2,and MaEXPA15)and starch degradation(MaGWD1 and MaLSF2),and these bindings were validated by EMSA and DLR assays.Transient overexpression and knockdown of MabHLH28 in banana fruit resulted in up-and down-regulation of softening-related genes,thereby hastening and postponing fruit ripening.Furthermore,overexpression of MabHLH28 in tomato accelerated the ripening process by elevating the accumulation of softening-associated genes.In addition,MabHLH28 showed interaction withMaWRKY49/111 and itself to form protein complexes,which could combinatorically strengthen the transcription of softening-associated genes.Taken together,our findings suggest that MabHLH28 mediates fruit softening by upregulating the expression of softening-related genes either alone or in combination with MaWRKY49/111.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation,Ministry of Education,College of Horticulture,South China Agricultural University,Guangzhou 510642,China
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