首页|Auxin regulates bulbil initiation by mediating sucrose metabolism in Lilium lancifolium

Auxin regulates bulbil initiation by mediating sucrose metabolism in Lilium lancifolium

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Lily bulbils,which serve as advantageous axillary organs for vegetative propagation,have not been extensively studied in terms of the mechanism of bulbil initiation.The functions of auxin and sucrose metabolism have been implicated in axillary organ development,but their relationship in regulating bulbil initiation remains unclear.In this study,exogenous indole-3-acetic acid(IAA)treatment increased the endogenous auxin levels at leaf axils and significantly decreased bulbil number,whereas treatment with the auxin polar transport inhibitor N-1-naphthylphthalamic acid(NPA),which resulted in a low auxin concentration at leaf axils,stimulated bulbil initiation and increased bulbil number.A low level of auxin caused by NPA spraying or silencing of auxin biosynthesis genes YUCCA FLAVIN MONOOXYGENASE-LIKE 6(LlYUC6)and TRYPTOPHAN AMINOTRANSFERASE RELATED 1(LlTAR1)facilitated sucrose metabolism by activating the expression of SUCROSE SYNTHASES 1(LlSusy1)and CELL WALL INVERTASE 2(LlCWIN2),resulting in enhanced bulbil initiation.Silencing LlSusy1 or LlCWIN2 hindered bulbil initiation.Moreover,the transcription factor BASIC HELIX-LOOP-HELIX 35(LlbHLH35)directly bound the promoter of LlSusy1,but not the promoter of LlCWIN2,and activated its transcription in response to the auxin content,bridging the gap between auxin and sucrose metabolism.In conclusion,our results reveal that an LlbHLH35-LlSusy1 module mediates auxin-regulated sucrose metabolism during bulbil initiation.

Yin Xin、Xi Chen、Jiahui Liang、Shaokun Wang、Wenqiang Pan、Jingxiang Wu、Mingfang Zhang、Michele Zaccai、Xiaonan Yu、Xiuhai Zhang、Jian Wu、Yunpeng Du

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Ornamental & Edible Lily Engineering Research Center of National Forestry and Grassland,Institute of Grassland,Flowers and Ecology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China

Beijing Key Laboratory of Development and Quality Control of Ornamental Crops,Department of Ornamental Horticulture and Landscape Architecture,China Agricultural University,Beijing 100193,China

College of Landscape Architecture,Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding,National Engineering Research Center for Floriculture,Beijing Laboratory of Urban and Rural Ecological Environment,Beijing Forestry University,Beijing 100083,China

Department of Life Sciences,Ben-Gurion University of the Negev,Beer Sheva 8410501,Israel

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国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金Excellent Youth Science Foundation of Beijing Academy of Agriculture and Forestry SciencesPinduoduo-China Agricultural University Research FundConstruction of Beijing Science and Technology Innovation and Service Capacity in Top Subjects中国农业大学"2115"人才培育发展支持工程项目Strategic Development Department of China Association for Science and Technology111 Project of the Ministry of Education中国博士后科学基金

3237195432171864323727403217261732302599YXQN202303PC2023B02009CEFF-PXM 2019_014207_000032B170432023M740311

2024

10.1093/hr/uhae054

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(4)
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