首页|Genome-wide methylation,transcriptome and characteristic metabolites reveal the balance between diosgenin and brassinosteroids in Dioscorea zingiberensis
Genome-wide methylation,transcriptome and characteristic metabolites reveal the balance between diosgenin and brassinosteroids in Dioscorea zingiberensis
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
Diosgenin(DG)is a bioactive metabolite isolated from Dioscorea species,renowned for its medicinal properties.Brassinosteroids(BRs)are a class of crucial plant steroidal hormones.Cholesterol and campesterol are important intermediates of DG and BR biosynthesis,respectively.DG and BRs are structurally similar components;however,the regulatory network and metabolic interplays have not been fully elucidated.In an effort to decode these complex networks,we conducted a comprehensive study integrating genome-wide methylation,transcriptome and characteristic metabolite data from Dioscorea zingiberensis.Leveraging these data,we were able to construct a comprehensive regulatory network linking DG and BRs.Mass spectrometry results enabled us to clarify the alterations in cholesterol,campesterol,diosgenin,and castasterone(one of the major active BRs).The DG content decreased by 27.72％at 6 h after brassinolide treatment,whereas the content increased by 85.34％at 6 h after brassinazole treatment.Moreover,we pinpointed DG/BR-related genes,such as CASs,CYP90s,and B3-ARFs,implicated in the metabolic pathways of DG and BRs.Moreover,CASs and CYP90s exhibit hypomethylation,which is closely related to their high transcription.These findings provide robust evidence for the homeostasis between DG and BRs.In conclusion,our research revealed the existence of a balance between DG and BRs in D.zingiberensis.Furthermore,our work not only provides new insights into the relationship between the two pathways but also offers a fresh perspective on the functions of secondary metabolites.
Zihao Li、Yi Li、Luyu Geng、Jiachen Wang、Yidan Ouyang、Jiaru Li
展开 >
State Key Laboratory of Hybrid Rice,College Life Sciences,Wuhan University,Wuhan 430072,China
National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research(Wuhan),Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,China
NETL
NSTL
万方数据
