首页|Transcriptomic analyses to summarize gene expression patterns that occur during leaf initiation of Chinese cabbage
Transcriptomic analyses to summarize gene expression patterns that occur during leaf initiation of Chinese cabbage
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
In Chinese cabbage,rosette leaves expose their adaxial side to the light converting light energy into chemical energy,acting as a source for the growth of the leafy head.In the leafy head,the outer heading leaves expose their abaxial side to the light while the inner leaves are shielded from the light and have become a sink organ of the growing Chinese cabbage plant.Interestingly,variation in several ad/abaxial polarity genes is associated with the typical leafy head morphotype.The initiation of leaf primordia and the establishment of leaf ad/abaxial polarity are essential steps in the initiation of marginal meristem activity leading to leaf formation.Understanding the molecular genetic mechanisms of leaf primordia formation,polar differentiation,and leaf expansion is thus relevant to understand leafy head formation.As Brassica's are mesa-hexaploids,many genes have multiple paralogues,complicating analysis of the genetic regulation of leaf development.In this study,we used laser dissection of Chinese cabbage leaf primordia and the shoot apical meristem(SAM)to compare gene expression profiles between both adaxial and abaxial sides and the SAM aiming to capture transcriptome changes underlying leaf primordia development.We highlight genes with roles in hormone pathways and transcription factors.We also assessed gene expression gradients along expanded leaf blades from the same plants to analyze regulatory links between SAM,leaf primordia and the expanding rosette leaf.The catalogue of differentially expressed genes provides insights in gene expression patterns involved in leaf development and form a starting point to unravel leafy head formation.
State Key Laboratory of North China Crop Improvement and Regulation,Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei,Ministry of Education of China-Hebei Province Joint Innovation Center for Efficient Green Vegetable Industry,College of Horticulture,Hebei Agricultural University,Baoding 071000,China
Plant Breeding,Wageningen University & Research,6708 PB Wageningen,The Netherlands
国家自然科学基金国家自然科学基金国家自然科学基金Dutch Royal Academy of Sciences China Exchange ProgramProject of Hebei Provincial Department of Human Resources and Social Security
NETL
NSTL
万方数据
