首页|Chromosome-scale reference genome of broccoli(Brassica oleracea var.italica Plenck)provides insights into glucosinolate biosynthesis

Chromosome-scale reference genome of broccoli(Brassica oleracea var.italica Plenck)provides insights into glucosinolate biosynthesis

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Broccoli(Brassica oleracea var.italica Plenck)is an important vegetable crop,as it is rich in health-beneficial glucosinolates(GSLs).However,the genetic basis of the GSL diversity in Brassicaceae remains unclear.Here we report a chromosome-level genome assembly of broccoli generated using PacBio HiFi reads and Hi-C technology.The final genome assembly is 613.79 Mb in size,with a contig N50 of 14.70 Mb.The GSL profile and content analysis of different B.oleracea varieties,combined with a phylogenetic tree analysis,sequence alignment,and the construction of a 3D model of the methylthioalkylmalate synthase 1(MAM1)protein,revealed that the gene copy number and amino acid sequence variation both contributed to the diversity of GSL biosynthesis in B.oleracea.The overexpression of BoMAM1(BolI0108790)in broccoli resulted in high accumulation and a high ratio of C4-GSLs,demonstrating that BoMAM1 is the key enzyme in C4-GSL biosynthesis.These results provide valuable insights for future genetic studies and nutritive component applications of Brassica crops.

Qiuyun Wu、Shuxiang Mao、Huiping Huang、Juan Liu、Xuan Chen、Linghui Hou、Yuxiao Tian、Jiahui Zhang、Junwei Wang、Yunsheng Wang、Ke Huang

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College of Horticulture,Hunan Agricultural University,Changsha,Hunan,410128,China

Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding,Ministry of Education,Changsha,Hunan,410128,China

Key Laboratory for Vegetable Biology of Hunan Province,Changsha,Hunan,410128,China

Hunan Provincial Key Laboratory for Biology and Control of Plant Disease and Insect Pests,Hunan Agricultural University,Changsha,Hunan,410128,China

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National Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaInternational Cooperation Projects of National Key R&D Program of ChinaGraduate Research Innovation Project of Hunaninnovation and entrepreneurship training program for college students

2022YFF1003000323726823227274732072585320725682022YFE01083002023XC103S202310537006X

2024

10.1093/hr/uhae063

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(5)