Integrated proteomic,transcriptomic,and metabolomic profiling reveals that the gibberellin-abscisic acid hub runs flower development in the Chinese orchid Cymbidium sinense

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外文摘要：The seasonal flowering Chinese Cymbidium produce an axillary floral meristem and require a dormancy period during cold conditions for flower development.However,the bud activation mechanism remains elusive.This study evaluates the multi-omics across six stages of flower development,along with functional analysis of core genes to decipher the innate mechanism of floral bud initiation and outgrowth in the Chinese orchid Cymbidium sinense.Transcriptome and proteome analyses identified 10 modules with essential roles in floral bud dormancy and activation.Gene clusters in the early stages of flower development were mainly related to flowering time regulation and meristem determination,while the late stages were correlated with hormone signaling pathways.The metabolome identified 69 potential hormones in which gibberellin(GA)and abscisic acid(ABA)were the main regulatory hubs,and GA4 and GA53 exhibited a reciprocal loop.Extraneous GA application caused rapid elongation of flower buds and promoted the expression of flower development genes.Contrarily,exogenous ABA application extended the dormancy process and ABA inhibitors induced dormancy release.Moreover,CsAPETALA1(CsAP1)was identified as the potential target of ABA for floral bud activation.Transformation of CsAP1 in Arabidopsis and its transient overexpression in C.sinense protoplasts not only affected flowering time and floral organ morphogenesis in Arabidopsis but also orchestrated the expression of flowering and hormone regulatory genes.The presence of ABA response elements in the CsAP1 promoter,rapid downregulation of CsAP1 after exogenous ABA application,and the activation of the floral bud after ABA inhibitor treatment suggest that ABA can control bud outgrowth through CsAP1.

作者：

Sagheer Ahmad、Chuqiao Lu、Jie Gao、Yonglu Wei、Qi Xie、Jianpeng Jin、Genfa Zhu、Fengxi Yang

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作者单位：

Guangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization,Environmental Horticulture Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China

Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510640,China

基金：

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaGuangzhou Science and Technology ProjectInnovation Team of Modern Agriculture Industry Technology System in Guangdong ProvinceGuangdong Academy of Agricultural sciences Discipline Team Construction ProjectGuangdong Academy of Agricultural sciences Discipline Team Construction ProjectGuangdong Academy of Agricultural sciences Discipline Team Construction Project

项目编号：

31872151316721842017070103072021KJ121202127TDBZ202006R2020PY-JX018

出版年：

2024

DOI：

10.1093/hr/uhae073

园艺研究(英文)

CSTPCD

ISSN：

年,卷(期)：2024.11(5)