首页|Calmodulin-like protein MdCML15 interacts with MdBT2 to modulate iron homeostasis in apple

Calmodulin-like protein MdCML15 interacts with MdBT2 to modulate iron homeostasis in apple

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BTB and TAZ domain proteins(BTs)function as specialized adaptors facilitating substrate recognition of the CUL3-RING ubiquitin ligase(CRL3)complex that targets proteins for ubiquitination in reaction to diverse pressures.Nonetheless,knowledge of the molecular mechanisms by which the apple scaffold protein MdBT2 responds to external and internal signals is limited.Here we demonstrate that a putative Ca2+ sensor,calmodulin-like 15(MdCML15),acts as an upstream regulator of MdBT2 to negatively modulate its functions in plasma membrane H+-ATPase regulation and iron deficiency tolerance.MdCML15 was identified to be substantially linked to MdBT2,and to result in the ubiquitination and degradation of the MdBT2 target protein MdbHLH104.Consequently,MdCML15 repressed the MdbHLH104 target,MdAHA8's expression,reducing levels of a specific membrane H+-ATPase.Finally,the phenotype of transgenic apple plantlets and calli demonstrated that MdCML15 modulates membrane H+-ATPase-produced rhizosphere pH lowering alongside iron homeostasis through an MdCML15-MdBT2-MdbHLH104-MdAHA8 pathway.Our results provide new insights into the relationship between Ca2+signaling and iron homeostasis.

Xiao-Juan Liu、Xin Liu、Qiang Zhao、Yuan-Hua Dong、Qiangbo Liu、Yuan Xue、Yu-Xin Yao、Chun-Xiang You、Hui Kang、Xiao-Fei Wang

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National Key Laboratory of Wheat Improvement,Apple Technology Innovation Center of Shandong Province,Shandong Green Fertilizer Technology Innovation Center,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai-An,271018,Shandong,China

State Key Laboratory of Tree Genetics and Breeding,Chinese Academy of Forestry,Beijing 100091,China

Institute of Forestry and Pomology,Academy of Agriculture and Forestry Sciences,Beijing 100093,China

College of Horticulture,Qingdao Agricultural University,Qingdao 266109,China

National Key Laboratory of Wheat Improvement,College of Life Sciences,Shandong Agricultural University,Tai-An,271018,China

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National Key Research and Development ProgramNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaChina Agriculture Research System of MOF and MARANatural Science Foundation of Shandong Province

2022YFD12017013200133632272683CARS-27ZR2022QC093

2024

10.1093/hr/uhae081

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(5)