首页|Genome-wide transcriptome analysis reveals key regulatory networks and genes involved in the determination of seed hardness in vegetable soybean

Genome-wide transcriptome analysis reveals key regulatory networks and genes involved in the determination of seed hardness in vegetable soybean

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Seed hardness is an important quality trait of vegetable soybean.To determine the factors underlying seed hardness,two landraces with contrasting seed hardness,Niumaohuang(low seed hardness)and Pixiansilicao(high seed hardness),were selected from 216 soybean accessions originating from 26 provinces in China.The contents of the main components in vegetable soybean seeds such as water,soluble sugar,starch,protein and oil were measured,and transcriptome analyses performed during five stages of seed developmental.Transcriptome analysis indicates that during the middle and late stages of seed development,a large number of genes involved in the synthesis or degradation of starch,storage protein,and fatty acids were differentially expressed,leading to differences in the accumulation of stored substances during seed maturation among Niumaohuang and Pixiansilicao.The activity of cell proliferation and the formation of cell walls in the middle and late stages of seed development may also affect the hardness of seeds to a certain extent.In addition,weighted gene co-expression network analysis(WGCNA)was undertaken to identify co-expressed gene modules and hub genes that regulate seed hardness.Overexpression of a candidate seed hardness regulatory hub gene,GmSWEET2,resulted in increased seed hardness.In this study,the important role of GmSWEET2 in regulating the hardness of vegetable soybean seeds was verified and numerous potential key regulators controlling seed hardness and the proportion of seed components were identified,laying the groundwork for improving the texture of vegetable soybean.

Congcong Wang、Jianyu Lin、Yuanpeng Bu、Ruidong Sun、Yang Lu、JunYi Gai、Han Xing、Na Guo、Jinming Zhao

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Key Laboratory of Biology and Genetics Improvement of Soybean,Ministry of Agriculture/Zhongshan Biological Breeding Laboratory(ZSBBL)/National Innovation Platform for Soybean Breeding and Industry-Education Integration/State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization/College of Agriculture,Nanjing Agricultural University,Nanjing 210095,China

Key Research and Development Program(Modern Agriculture)of Jiangsu ProvinceNational Key R&D Program of ChinaZhongshan Biological Breeding Laboratory(ZSBBL)Zhongshan Biological Breeding Laboratory(ZSBBL)'JBGS' Project of Seed Industry Revitalization in Jiangsu ProvinceChina Agriculture Research System of MOF and MARANational Natural Science Foundation of ChinaFundamental Research Funds for the Central UniversitiesJiangsu Agriculture Science and Technology Innovation FundJiangsu Agriculture Science and Technology Innovation FundKey R&D project of Jiangsu ProvinceJiangsu Collaborative Innovation Center for Modern Crop Production and Cyrus Tang Innovation Center for Seed Industry

BE20233482021YFD1201605BM2022008-01ZSBBL-KY2023-03JBGS2021059CARS-0431471519XUEKEN2023022CX225004CX202015BE2019376

2024

10.1093/hr/uhae084

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(5)