首页|Identification of allelic relationship and translocation region among chromosomal translocation lines that leads to less-seed watermelon

Identification of allelic relationship and translocation region among chromosomal translocation lines that leads to less-seed watermelon

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Less-seed and seedless traits are desirable characteristics in watermelon(Citrullus lanatus).Hybridization between watermelon chromosomal translocated lines and wild lines significantly reduced seed counts in the hybrid fruits,approaching even seedless.However,the allelic relationships and the chromosomal translocation breakpoints from different sources are unclear,which limits their utility in breeding practices.This study focused on three groups of chromosomal translocation materials from different sources and conducted inheritance and allelic relationship analysis of translocation points.The results from third-generation genome sequencing and fluorescence in situ hybridization(FISH)revealed that the specific translocations in the naturally mutated material MT-a involved reciprocal translocations between Chr6 and Chr10.The Co60y radiation-induced mutant material MT-b involved reciprocal transloca-tions between Chr1 and Chr5,Chr4 and Chr8.The Co60y radiation-induced mutant material MT-c involved complex translocations among Chr1,Chr5,and Chr11.Cytological observation showed that heterozygous translocation hybrids showed chromosomal synapsis abnormalities during meiotic diakinesis.Further,dominant and codominant molecular markers were developed on both sides of the translocation breakpoints,which could facilitate rapid and efficient identification of chromosome translocation lines.This study provides technical guidance for utilizing chromosomal translocation materials in the development of less-seed watermelon varieties.

Di Jiao、Hong Zhao、Honghe Sun、Jie Zhang、Haiying Zhang、Guoyi Gong、Muhammad Anees、Hongju Zhu、Wenge Liu、Yong Xu

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National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops,Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Hanghai East Road,Guancheng District,Zhengzhou,Henan 450009,China

State Key Laboratory of Vegetable Biobreeding,Tianjin Academy of Agriculture Sciences,Jinjing Road,Xiqing District,Tianjin 300192,China

State Key Laboratory of Vegetable Biobreeding,National Engineering Research Center for Vegetables,Beijing Key Laboratory of Vegetable Germplasms Improvement,Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Science,Zhanghua Road,Haidian Districk,Beijing 100097,China

Plant Biology Section,School of Integrative Plant Science,Cornell University,236 Tower Road,Ithaca,New York 14853,USA

Boyce Thompson Institute,533 Tower Road,Ithaca,New York 14853,USA

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National Natural Science Foundation of ChinaMinistry of Agriculture and Rural Affairs of ChinaScientist Training Program of BAAFSCollaborative Innovation Center of BAAFSInnovation and Development Program of BVRCNational Key Research and Development Program of Chinaagricultural Science,and Technology Innovation Program

32330093CARS-25JKZX202401KJCX201907-2KYCX2024012023YFF1000100CAAS-ASTIP-2021-ZFRI

2024

10.1093/hr/uhae087

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(5)