首页|A CRISPR/Cas9-induced male-sterile line facilitating easy hybrid production in polyploid rapeseed(Brassica napus)
A CRISPR/Cas9-induced male-sterile line facilitating easy hybrid production in polyploid rapeseed(Brassica napus)
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Rapeseed is a globally significant oilseed crop cultivated to meet the increasing demand for vegetable oil.In order to enhance yield and sustainability,breeders have adopted the development of rapeseed hybrids as a common strategy.However,current hybrid production systems in rapeseed have various limitations,necessitating the development of a simpler and more efficient approach.In this study,we propose a novel method involving the targeted disruption of Defective in Anther Dehiscence1 of Brassica napus(BnDAD1),an essential gene in the jasmonic acid biosynthesis pathway,using CRISPR/Cas9 technology,to create male-sterile lines.BnDAD1 was found to be dominantly expressed in the stamen of rapeseed flower buds.Disrupting BnDAD1 led to decreased levels of α-linolenic acid and jasmonate in the double mutants,resulting in defects in anther dehiscence and pollen maturation.By crossing the double mutant male-sterile lines with male-fertile lines,a two-line system was demonstrated,enabling the production of F1 seeds.The male-sterile trait of the bndad1 double mutant lines was maintainable by applying exogenous methyl jasmonate and subsequently self-pollinating the flowers.This breakthrough holds promising potential for harnessing heterosis in rapeseed and offers a simpler and more efficient method for producing hybrid seeds.
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