JrPHL8-JrWRKY4-JrSTH2L module regulates resistance to Colletotrichum gloeosporioides in walnut

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外文摘要：Walnut anthracnose(Colletotrichum gloeosporioides)reduces walnut yield and quality and seriously threatens the healthy development of the walnut industry.WRKY transcription factors(TFs)are crucial regulatory factors involved in plant-pathogen interactions.Our previous transcriptome analysis results indicate that JrWRKY4 responds to infection by C.gloeosporioides,but its specific regulatory network and disease resistance mechanism are still unclear.Herein,the characteristics of JrWRKY4 as a transcription activator located in the nucleus were first identified.Gain-of-function and loss-of-function analyses showed that JrWRKY4 could enhance walnut resistance against C.gloeosporioides.A series of molecular experiments showed that JrWRKY4 directly interacted with the promoter region of JrSTH2L and positively regulated its expression.In addition,JrWRKY4 interacted with JrVQ4 to form the protein complex,which inhibited JrWRKY4 for the activation of JrSTH2L.Notably,a MYB TF JrPHL8 interacting with the JrWRKY4 promoter has also been identified,which directly bound to the MBS element in the promoter of JrWRKY4 and induced its activity.Our study elucidated a novel mechanism of the JrPHL8-JrWRKY4-JrSTH2L in regulating walnut resistance to anthracnose.This mechanism improves our understanding of the molecular mechanism of WRKY TF mediated resistance to anthracnose in walnut,which provides new insights for molecular breeding of disease-resistant walnuts in the future.

作者：

Yutian Mu、Yuhui Dong、Xichen Li、Andi Gong、Haiyi Yu、Changxi Wang、Jianning Liu、Qiang Liang、Keqiang Yang、Hongcheng Fang

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作者单位：

College of Forestry,Shandong Agricultural University,Taian 271018,Shandong,China

Mountain Tai Forest Ecosystem Research Station of State Forestry and Grassland Administration,Shandong Agricultural University,Taian 271018,Shandong,China

State Forestry and Grassland Administration Key Laboratory of Silviculture in Downstream Areas of the Yellow River,Taian 271018,Shandong,China

出版年：

2024

DOI：

10.1093/hr/uhae148

园艺研究(英文)

CSTPCD

ISSN：

年,卷(期)：2024.11(7)