首页|The tomato WRKY-B transcription factor modulates lateral branching by targeting BLIND,PIN4,and IAA15

The tomato WRKY-B transcription factor modulates lateral branching by targeting BLIND,PIN4,and IAA15

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Lateral branching is a crucial agronomic trait that impacts crop yield.In tomato(Solanum lycopersicum),excessive lateral branching is unfavorable and results in substantial labor and management costs.Therefore,optimizing lateral branching is a primary objective in tomato breeding.Although many genes related to lateral branching have been reported in tomato,the molecular mechanism underlying their network remains elusive.In this study,we found that the expression profile of a WRKY gene,WRKY-B(for WRKY-BRANCING),was associated with the auxin-dependent axillary bud development process.Wrky-b mutants generated by the CRISPR/Cas9 editing system presented fewer lateral branches,while WRKY-B overexpression lines presented more lateral branches than did wild-type plants.Furthermore,WRKY-B can directly target the well-known branching gene BLIND(BL)and the auxin efflux carrier gene PIN4 to activate their expression.Both the bl and pin4 mutants exhibited reduced lateral branching,similar to the wrky-b mutant.The IAA contents in the axillary buds of the wrky-b,bl,and pin4 mutant plants were significantly higher than those in the wild-type plants.In addition,WRKY-B can also directly target the AUX/IAA gene IAA15 and repress its expression.In summary,WRKY-B works upstream of BL,PIN4,and IAA15 to regulate the development of lateral branches in tomato.

Huanhuan Yang、Ke Zhou、Qingfei Wu、Xinyi Jia、Hexuan Wang、Wenhui Yang、Lihao Lin、Xiaomeng Hu、Bingqing Pan、Ping Li、Tingting Huang、Xiangyang Xu、Jingfu Li、Jingbin Jiang、Minmin Du

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College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,China

College of Horticulture,China Agricultural University,Beijing 100083,China

College of Life Sciences,Yan'an University,Yan'an 716000,China

College of Agriculture,Ningxia Universisty,Yinchuan 750002,China

Qingdao Academy of Agricultural Sciences,Qingdao City 266000,China

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2024

10.1093/hr/uhae193

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(9)