首页|Integrating QTL mapping and transcriptomics to decipher the genetic architecture of sterol metabolism in Brassica napus L

Integrating QTL mapping and transcriptomics to decipher the genetic architecture of sterol metabolism in Brassica napus L

扫码查看
原文链接
  • 万方数据
Sterols are secondary metabolites commonly found in rapeseed that play crucial physiological roles in plants and also benefit human health.Consequently,unraveling the genetic basis of sterol synthesis in rapeseed is highly important.In this study,21 individual sterols as well as total sterol(TS)content were detected in a double haploid(DH)population of Brassica napus,and a total of 24 quantitative trait loci(QTL)and 157 mQTL were identified that were associated with TS and different individual sterols.Time-series transcriptomic analysis showed that the differentially expressed genes(DEGs)involved in sterol and lipid biosynthesis pathways were enriched.Additionally,a regulatory network between sterol-related DEGs and transcription factors(TFs)was established using coexpression analysis.Some candidate genes were identified with the integration of transcriptomic analysis and QTL mapping,and the key candidate gene BnSQS1.C03 was selected for further functional analysis.BnSQS1.C03 demonstrated squalene synthase activity in vitro and increased the TS by 3.8％when overexpressed in Arabidopsis.The present results provide new insights into sterol regulatory pathways and a valuable genetic basis for breeding rapeseed varieties with high sterol content in the future.

Yiyi Xiong、Guangyuan Lu、Huaixin Li、Jianjie He、Shipeng Fan、Shuxiang Yan、Liangxiao Zhang、Haibo Jia、Maoteng Li

展开 >

Key Laboratory of Molecular Biophysics of the Ministry of Education,College of Life Science and Technology,Huazhong University of Science and Technology,1037 Luoxiong Road,Hongshan District,Wuhan 430074,China

College of Biology and Food Engineering,Kechuang 1st Road,Maonan District,Guangdong University of Petrochemical Technology,Maoming 525000,China

Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Xudong 2nd Road,Wuchang District,Wuhan 430062,China

2024

10.1093/hr/uhae196

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(9)