首页|Visualization of metabolite distribution based on matrix-assisted laser desorption/ionization-mass spectrometry imaging of tea seedlings(Camellia sinensis)

Visualization of metabolite distribution based on matrix-assisted laser desorption/ionization-mass spectrometry imaging of tea seedlings(Camellia sinensis)

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Tea seedlings(Camellia sinensis)have a well-developed root system with a strong taproot and lateral roots.Compared with ordinary cuttings,tea has stronger vitality and environmental adaptability,thus facilitating the promotion of good varieties.However,there is less of detailed research on the rooting and germination process of tea seeds.In this study,matrix-assisted laser desorption ionization time-of-flight-mass spectrometry was used to conduct non-targeted spatial mass spectrometry imaging of the main organs during growth of tea seedlings.A total of 1234 compounds were identified,which could be divided into 24 classes.Among them,theanine,as the most prominent nitrogen compound,was synthesized rapidly at the early stage of embryo germination,accounting for＞90％of the total free amino acids in the radicle,and it was then transferred to each meristem region through the mesocolumnar sheath,indicating that theanine-based nitrogen flow plays a decisive role in organ formation during the development of tea seedlings.Nutrients stored in the cotyledon were rapidly hydrolyzed to dextrin and 3-phosphoglyceraldehyde at the early stages of germination,and subsequently converted to other forms that provided carbon and energy for development,such as raffinose and D-galactose(glucose),which were mainly distributed in the growing zones of the root apex and the apical meristems of the stem.This study provides a new perspective on the synthesis and metabolism of substances during the development of tea seedlings and contributes to a better understanding of the biological characteristics of tea varieties.

Maoyin Fu、Liying Tian、Dongqiao Zheng、Yang Gao、Chenyi Sun、Shihua Zhang、ZhaoLiang Zhang、Xiaochun Wan、Qi Chen

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State Key Laboratory of Tea Plant Biology and Utilization,School of Tea and Food Science & Technology,Anhui Agricultural University,Hefei 230036,China

College of Computer Science,South-Central Minzu University,Wuhan 430074,China

Key Laboratory of Food Nutrition and Safety,Anhui Engineering Laboratory for Agro-products Processing,School of Tea and Food Science & Technology,Anhui Agricultural University,Hefei 230036,China

2024

10.1093/hr/uhae218

园艺研究(英文)

园艺研究(英文)

CSTPCD
ISSN：
年,卷(期)：2024.11(10)