Fine mapping and identification of candidate genes associated with powdery mildew resistance in melon(Cucumis melo L.)

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外文摘要：Powdery mildew(PM),a common disease of many major crop species,including melon(Cucumis melo L.),affects plant growth and fruit quality and seriously reduces production.Using a combined morphological and molecular approach,we attribute the PM pathogen that naturally occurs in melon to Podosphaera xanthii,and specifically to physiological race 1.An investigation into the genetic basis of PM resistance in melon using the resistant accession'PI 164637'and susceptible counterpart'HDZ'reveals dominant inheritance of PM resistance at the seedling stage,supported by F2 and backcross population segregation ratios.Adult plant assessments indicate a major gene with an additive effect for PM resistance.Bulk segregant analysis coupled with high-throughput sequencing identified a significant quantitative trait locus on chromosome 6 that is associated with PM resistance.Genetic mapping narrowed down the candidate region to 63.5 kb using InDel molecular markers,harboring 12 candidate genes.The marker chr06_indel_5 047127 demonstrated high accuracy in screening PM resistance in an F2 segregating population and 30 inbred lines as natural populations.Functional annotation and expression analysis of candidate genes revealed that MYB transcription factor MELO3C006700,GATA transcription factor MELO3C028829 and heparanase-like protein MELO3C006697 are promising candidate genes for PM resistance in melon.The genetic architecture underlying this resistance in melon offers valuable insights for breeding programs,and the identified markers,especially chr06_indel_5 047127,may enable practical applications for marker-assisted selection in developing PM-resistant melon varieties.

作者：

Xiaoyu Duan、Yue Yuan、Núria Real、Mi Tang、Jian Ren、Jiaqi Wei、Bin Liu、Xuejun Zhang

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作者单位：

Hami-melon Research Center,Xinjiang Academy of Agricultural Sciences,Nanchang Road 403,Saybagh District,Urumqi 830091,China

Sanya Mingzhu Melon and Watermelon Variety Demonstration Evaluation and Research Center,Yazhou District,Sanya 572000,China

Wuhan Academy of Agricultural Sciences,Baishazhou Avenue 107,Hongshan District,Wuhan 430072,China

Plant Pathology,IRTA Cabrils.Carretera de Cabrils km 2,08348 Cabrils Spain

Anhui Jianghuai Horticulture Seeds Co.,Ltd,Changfeng County,Hefei City,Anhui Province

Hainan Sanya Crops Breeding Trial Center of Xinjiang Academy Agricultural Sciences,Haitang District,Sanya,572000

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出版年：

2024

DOI：

10.1093/hr/uhae222

园艺研究(英文)

CSTPCD

ISSN：

年,卷(期)：2024.11(10)