Molecular characteristics of OmpP2 gene in Glaesserella parasuis and construction and identification of its recombinant lactic acid bacteria
Glässer's disease is a major bacterial disease affecting the development of pig farming.Constructing a novel vaccine that can safely and effectively control the disease has been a hot topic in epidemic prevention and control research.In this study,following bioinformatics prediction and analysis,we constructed the pNZ8148-OmpP2 recombinant vector by ligating the OmpP2 gene into the pNZ8148 vector utilizing seamless cloning technolo-gy.Then transfected the recombinant vector into Lactococcus lactis receptor cells to obtain pNZ8148-OmpP2 re-combinant Lactococcus lactis.The protein expression and characterization of the recombinant bacteria were then performed.The results indicate that the OmpP2 protein has advantageous B and T cell epitopes which are potential candidate antigen.The recombinant Lactobacillus was successfully detected a target band with the size of approxi-mately 38.5 kD,which was consistent with the expected size,indicating that Lactococcus lactis could be used as a delivery vector to express G.parasuis heterologous protein successfully.Our research laid the foundation for the subsequent development of the live recombinant Lactococcus lactis vaccine for G.parasuis.
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维普
