Aiming at the rapid quantitative detection of trimethylamine oxide(TMAO)in seafood,an electrochemical analysis method using microfluidic photochemical reduction chip for pretreatment is proposed.Firstly,the PDMS/SiO2 hybrid microfluidic chip is fabricated,and the pre-treatment reaction reduction conditions of TMAO are optimized.Using this microfluidic chip and Fe(Ⅱ)-HEDP as reducing agent,under the conditions of pH 6.5,temperature 40℃ and UV-irradiation time 2 min,the reduction rate of TMAO to trimethylamine(TMA)can reach 99.44％.Then,the self-made selective electrode is used to detect TMA solution.In the range of 0.01～10 mmol·L-1,the logarithm of TMA concentration is linearly correlated with the solution potential value,the cor-relation coefficient r2 is 0.997 7,and the detection limit is 2.28 μmol·L-1.The relative deviation of this method for TMAO detection of actual seafood products is not more than 4.71％,and the recovery rate is 91.78％～112.16％.The method has the advantages of short determination time and accurate results,and shows a good application prospect in the analysis and detection of seafood.
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