Brucellosis is one of the zoonotic diseases of animal origin that seriously threatens human health.The prevention and control of brucellosis in animals is mainly based on prevention.At present,there are S2,A19 and M5 vaccines for brucellosis in China,and S19 and Rev.1 vaccines in the world,among which M5 and Rev.1 strains of sheep are the least stable.TSA is Tryptone soy agar medium(also known as Tryptone soy agar medium),a commercial medium that is suitable for the growth of Brucella Rev.1 strain.After TSA medium plate with different agar concentration was placed under different conditions(temperature and time),Brucella Rev.1 strain was inoculated.After 96 h culture,colony growth was observed and colony crystal violet staining was performed.Through comparative analysis,it is found that the humidity of TSA medium plate is an important factor affecting the accuracy of variation test results.At the same time,the preparation method of TSA medium for Brucella Rev.1 strain was clarified.
Brucella Rev.1agar concentrationtemperature and timevariation rate
国家科技期刊平台
NSTL
维普
万方数据
