猫血型荧光PCR检测方法的建立及应用
Establishment and Application of Real-Time PCR for Detection of Feline Blood Type
赵方圆 1巩玉洁 1赵荣茂 1邓西 1毕铭钰1
作者信息
- 1. 北京纳百生物科技有限公司,北京 102600
- 折叠
摘要
猫的AB型血型系统由A型、B型和AB 3种血型组成.配型不合可能导致急性溶血性输血反应和新生儿溶血.根据TaqMan探针荧光定量分析原理,通过对猫血型决定基因胞苷-磷酸-N-乙酰神经氨酸羟基酶(CMAH)基因的4个血型相关的位点进行序列比对,设计相应引物以及探针,建立猫血型荧光PCR检测方法.该研究建立的猫血型荧光PCR检测方法与商品化的血型检测试纸条检测结果符合度达100％.此外,研究发现在北京及周边地区采集的269个猫样本中,A型血型占94.05％、B型占5.58％、AB型占0.37％.
Abstract
The AB blood type system in feline is composed of three blood types:A,B and AB.Mismatches may result in acute hemolytic transfusion reactions and neonatal isoerythrolysis.According to the principle of TaqMan probe fluorescence quantitative analysis.four sites of the feline blood type determining gene,cytidine-phospho-N-acetylneuraminic acid hydroxylase(CMAH)gene,were aligned,and the corresponding primers and probes were designed to establish a real-time PCR detection method for feline blood type.The real-time PCR method for feline blood type detection established in this study was 100％consistent with the results of Alvedia rapid-test(LabTest A+B).In addition.in the 269 feline samples collected in Beijing and surrounding areas,the proportions of A,B and AB blood type were 94.05％,5.58％and 0.37％,respectively.
关键词
猫血型/CMAH基因/基因型检测/荧光PCRKey words
Feline blood type/CMAH gene/Detection of genotypes/real-time PCR引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据