Establishment and Application of Real-Time PCR for Detection of Feline Blood Type
The AB blood type system in feline is composed of three blood types:A,B and AB.Mismatches may result in acute hemolytic transfusion reactions and neonatal isoerythrolysis.According to the principle of TaqMan probe fluorescence quantitative analysis.four sites of the feline blood type determining gene,cytidine-phospho-N-acetylneuraminic acid hydroxylase(CMAH)gene,were aligned,and the corresponding primers and probes were designed to establish a real-time PCR detection method for feline blood type.The real-time PCR method for feline blood type detection established in this study was 100％consistent with the results of Alvedia rapid-test(LabTest A+B).In addition.in the 269 feline samples collected in Beijing and surrounding areas,the proportions of A,B and AB blood type were 94.05％,5.58％and 0.37％,respectively.
Feline blood typeCMAH geneDetection of genotypesreal-time PCR
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