Study on the Expression of Chicken W Chromosome Gene CTIF in Chicken Embryo Tissues
In this paper,the expression of CTIF during embryonic development was studied.By designing primers(CTIF-W,CTIF-Z and CTIF-ZW)that specifically amplify W and Z copies and simultaneously amplify W and Z copies,the mRNA expression levels of W and Z copies in different day-old tissues of embryos were analyzed,and the response to gender phenotype was explored by FAD(fadrozole)induced reversal.The results showed that the expression of CTIF-W in the gonads of embryos at 6 days of incubation was higher than that in the tail,and the expression level gradually increased with embryonic development,while the expression trend of CTIF-Z and CTIF-ZW was similar.In other tissues of 6-day-old chicken embryos,the expression of CTIF-ZW in kidney was significantly higher than that in other tissues,indicating that CTIF may also be related to the development or function of kidney while exerting certain basic functions.The expression of CTIF-Z in most tissues was higher in males than in females,while the expression difference of CTIF-ZW between males and females was relatively small,indicating that W copy may regulate the expression of Z copy in females to a certain extent.Both CTIF-W and CTIF-Z were expressed in 12-day embryonic tissues and were highly expressed in spleen tissues.The expression of CTIF-Z in male was higher than that in female in most tissues.In addition,the expression of CTIF-W was significantly reduced by the effect of female-to-male sex reversal,while CTIF-Z did not respond.In summary,CTIF is highly expressed in the kidney and spleen of chicken embryos,and its Z copy has a dose effect between male and female tissues,while W copy may be used as a compensation for Z copy in female to ensure its biological function.
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