Prokaryotic Expression and Polyclonal Antibody Preparation of UL56 Protein of Equid Alphaherpesvirus 1
The purpose of this study is to prepare polyclonal antibody of pUL56 of Equid alphaherpesvirus 1.Based on the whole genome sequence of EHV-1 YM2019 strain in GenBank.The pUL56 was purified by Ni-NTA affinity chromatography,and BALB/c mice were immunized with Freund's adjuvant and pUL56 polyclonal antibody was prepared.The polyclonal antibody of the protein was measured by enzyme linked immunosorbent assay and Western blot.The results showed that the purified pUL56 could react with EHV-1 positive serum.The anti-pUL56 polyclonal antibody had a potency of 1∶128 000 and bound specifically to pUL56.In this study,the mouse anti-pUL56 polyclonal antibody had good reactogenicity,which laid the theoretical foundation for functional properties of pUL56 and the pathogenic mechanisms of virus.
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