Analysis of the Flavonoids Content of Anoectochilus roxburghii in Zhaoqing under Tissue Culture and Greenhouse Cultivation
To analyze the extraction technology of total flavonoids from Zhaoqing Anoectochilus roxburghii under tissue culture and greenhouse cultivation based on ethanol extraction method and orthogonal experiment,and to compare the changes in the total flavonoids content of Anoectochilus roxburghii under different growth cycles.The ethanol extraction method was used to examine the effects of ethanol concentration,liquid-to-material ratio,and extraction temperature on the Anoectochilus roxburghii flavonoid in different planting methods.Then the optimal extraction process was analyzed through orthogonal experiments,and after verification,this condition was used to determine the Anoectochilus roxburghii flavonoid of different months.The optimal combination of the Anoectochilus roxburghii flavonoid was a solid-liquid ratio of 1∶30 g/mL,a water bath temperature of 70℃,and an ethanol concentration of 80%.The yield of total flavonoids in the verification test was 1.926%(RSD=1.11%,R2=0.992).The total flavonoid content of the Anoectochilus roxburghii is related to the month.As the month increases,the total flavonoid content of Anoectochilus of the two planting methods gradually increases.And reached the highest value at the 7th month.The optimized ethanol extraction process can stably extract total flavonoids from Zhaoqing Anoectochilus roxburghii under different planting methods,and reach the maximum value in the 7th month,which provides a reference for the analysis of active ingredients of Anoectochilus roxburghii and the quality evaluation of flavonoids.
国家科技期刊平台
NSTL
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