阿留申病毒阳性水貂选种简易方法的建立与效果评估
Establishment and Effect Evaluation of a Simple Method for Selecting Aleutian Virus-positive Mink
张旭 1吴晓艳 1曹章 1于天浩 1赵静 1蒋欣芫 1孙见 2张彦龙3
作者信息
- 1. 东北林业大学野生动物与自然保护地学院,黑龙江 哈尔滨 150006
- 2. 威海海洋职业学院,山东荣成 264315;河北农业大学,河北 保定 071000
- 3. 东北林业大学野生动物与自然保护地学院,黑龙江 哈尔滨 150006;威海海洋职业学院,山东荣成 264315
- 折叠
摘要
通过从阿留申病毒阳性水貂筛选具有抵抗力的水貂,达到抗病毒育种的目的.第1年选留种后通过临床观察,把不健康水貂直接淘汰.第2年选种方法,除临床表现不健康的水貂淘汰外,趾尖采血,淘汰血浆比例高于50%以上水貂;第3年,除第2年的方法外,应用血清折光仪测定血浆中总蛋白的值,按35%比例从高往低淘汰.3年时间不同选种方法对比,逐年检测和淘汰,通过红细胞和血浆比例筛查及血清折光仪检测后,水貂产仔数接近阿留申病毒阴性水貂,可以应用此法逐年筛选出抗阿留申病毒的水貂种群,提高产仔数减少农场的损失.
Abstract
The purpose of virus-resistant breeding was achieved by screening resistant mink from Aleutian virus-positive mink.In the first year,the unhealthy mink was directly eliminated through clinical observation.In the second year,in addition to the elimination of mink with unhealthy clinical manifestations,blood was collected from the tip of the toe,and the proportion of plasma eliminated was higher than 50%mink.In the third year,in addition to the method of the second year,the value of total protein in plasma was determined by serum refractometer,and the proportion of 35%was eliminated from high to low.After comparing different selection methods in three years,testing and eliminating them year by year,after screening the proportion of red blood cells and plasma and testing by serum refractometer,the litter size of mink is close to that of Aleutian virus-negative mink,and this method can be used to screen out the Aleutian virus-resistant mink population year by year,increase the litter size and reduce the loss of the farm.
关键词
水貂阿留申病/血浆总蛋白/血清折光仪/选种评估Key words
Mink Aleutian disease/total plasma protein/serum refractometer/selection evaluation引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据