Establishment of Ear Marginal Tissue Fibroblast Cell Line of Matou Goat
Matou goat is a local excellent breed in the Wuling Mountains,known for its large size,excellent meat and skin quality,and is a key protected variety in Hubei Province.This study selected 1-year-old Matou goats of different genders as the experimental subjects,established a fibroblast cell line of Matou goat ear tissue using fiber protease digestion method,analyzed and compared the biological characteristics such as cell morphology,growth curve,survival rate before and after cryopreservation.The results showed that after 48 hours of cultivation,many typical fibroblast shaped cells could be observed surrounding the tissue block for growth.The cell doubling time was about 24 hours,and the recovery rates after cell cryopreservation were 96.0%and 96.5%respectively.The cell growth showed a typical"S"shape.The establishment of this cell line provides an ideal experimental material for the protection of genetic resources,and other basic research of Matou goats.
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