In order to rapidly detect Listeria monocytogenes in import and export large and medium-sized animals,the bacteriological method and the real-time fluorescence PCR detection method were combined,and the characteristics of propidium azide bromide(PMA)to inhibit the amplification of dead bacterial nucleic acid were used to pretreat the samples,and a set of PMA-qPCR real-time fluorescence rapid detection method for Listeria monocytogenes with infectious monocytogenes in import and export large and medium-sized animals was constructed.
关键词
进出境动物检疫/单核细胞增生李斯特氏菌/实时荧光PCR
Key words
exit and entry animal quarantine/Listeria monocytogenes/real-time fluorescence PCR
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