Preparation and Characteristics of NO2--N Degradation Compound Microbial Agent in Aquaculture Water
With the increase of the scale of aquaculture in China year by year,the problem of nitrogen compound accumulation in aquaculture water is becoming more and more serious.In order to realize the safe production of aquatic industry in China,a NO2--N degrading bacteria were screened out and compounded with photosynthetic bacteria and lactic acid bacteria to prepare a composite bacterial agent.The effects of temperature,pH,initial concentration of nitrite and inoculation amount on the degradation of NO2--Nwere analyzed by single factor experiments,and the optimal degradation effect and growth state of the compound microbial agent under different influencing factors were determined.The results showed that one strain A3 with high NO2--N oxidation properties was screened from the activated sludge and mixed with photosynthetic bacteria(Rhodopseudomonas palustris)and lactic acid bacteria(Lactobacillus acidophilus)according to 32∶2∶1,and the degradation efficiency was the highest.Under the conditions of 40℃,pH 9.0 and pH 6.0,the OD600 was stable at 0.2～0.4,and the degradation rate of NO2--N was maintained at about 50%.The compound microbial agent had the best growth and degradation rate at 25℃and pH 7.5.The degradation rate of the compound agent decreased with the increase of the initial NO2--N concentration,and the OD600 did not change significantly,indicating that the compound agent had a strong tolerance to NO2--N,and could maintain the degradation activity and maintain a certain degradation rate under the condition of 0.1～8.0 g/L.
compound microbial agentNO2--Naquaculture waterNO2--N high oxidizing effective bacteria
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