Whole-genome Sequencing of a Multidrug-resistant Strain of Escherichia coli Derived from Sika Deer
This experiment sequenced the entire genome of a strain of Escherichia coli from Sika deer with multiple drug resistance in the drug sensitivity test,and annotated the gene function of the strain using databases such as GO,KEGG,VFDB,CARD,etc.Draw a genome circular map using Circos software.The sequencing results showed that the total gene length of the strain was 4 838 417 bp,with a GC content of 50.78%.Contains a plasmid with a length of 93 378 bp.The predicted number of coding genes for this strain is 4 514,with a total length of 4 268 565 bp.It is predicted that this strain contains 14 gene islands.Gene functional analysis shows that the genome of this strain contains 3 208 genes corresponding to the KEGG database,of which 2 009 genes are involved in 49 signaling pathways.Through database comparison,it was found that there are 61 resistance genes in the genome of this strain,which are resistant to 16 types of antibiotics,and 898 virulence genes.The results of this experiment provide certain data reference for the research and prevention and control of antibiotic resistance in Escherichia coli.