The Effect of Lactobacillus reuteri Extracellular Vesicles on LPS-induced Inflammation of Bovine Endometrial Epithelial Cells
The aim of this study was to investigate the effect of Lactobacillus reuteri extracellular vesicles on LPS-induced inflammation in bovine endometrial epithelial cells.The outer vesicles of lactic acid bacteria were extracted by ultracentrifugation and representation was characterized.The intake of BEEC was detected by PKH-26 labeled outer vesicles.RT-qPCR WB and ELISA were used to detect the effects of EVs on the levels of PCNA,TNF-α,IL-1β,IL-6,IL-10 and NF-κB in LPS-induced BEEC inflammatory response.The result display that BEEC could ingest the outer vesicles of lactic acid bacteria,and the outer vesicles could promote the proliferation of BEEC.In LPS-induced BEEC cell inflammation,EVs can effectively inhibit the release of TNF-α,IL-β,IL-6 and NF-κB phosphorylation levels,and can promote the release of IL-10.Lactobacillus reuteri outer vesicles could alleviate BEEC cell inflammation by regulating the release of inflammatory factors.
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