ssc-miR-196a Affects Porcine Skeletal Muscle Development by Regulating PPARGC1A Gene Expression
In this study,two online programs,miRsystem and MiRWalk,were used to screen microRNAs that have potential regulatory relationships with the PPARGC1A gene.The reporter vectors psiCHECK2-PPARGC1A-3'UTR-WTand psiCHECK2-PPARGC1A-3'UTR-MUT were constructed,and the dual-luciferase assay was used to detect the target-regulatory relationship between the candidate microRNAs and PPARGC1A gene.The results revealed a potential regulatory relationship between ssc-miR-196a and the PPARGC1A gene.Dual luciferase assay showed that miR-196a could bind to the 3'UTR of PPARGC1A and significantly reduced luciferase activity in cells(P＜0.01).The expression level of ssc-miR-196a in longissimus muscle of Jinhua pigs at age 90 and 180 days was slightly higher than that of Large White pigs,and its expression level in longissimus muscle of pigs at age 180 days was significantly higher than that of pigs at age 30 and 90 days(P＜0.05).The specific binding of ssc-miR-196a to the 3'UTR of PPARGC1A,which provides a reference for further research into the regulation of porcine skeletal muscle development by microRNA-mediated PPARGC1A gene.
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