Intestinal Structure and Zoing Characteristics of Largemouth Bass
To understand the intestinal structure and zoning characteristics of largemouth bass,the morphological and histological characteristics of intestine were studied using anatomical and paraffin sectioning microscopy techniques.The intestine of largemouth bass was"S"-shaped,and the ratio of intestine length to body length was 0.76±0.06.HE-staining showed the intestinal tissue was composed of mucosal layer,submucosa,muscular layer and adventitia.The diameter of intestinal canal,the height and number of intestinal villi gradually decreased from front to back.The diameter and villus height of the anterior segment of the intestine were the longest,with an average diameter and height of(5043.60±108.69)μm and(1479.86±45.84)μm,respectively;The diameter and villus height of the distal end of the hindgut were the shortest,with values of(2877.10±57.21)μm and(790.28±62.57)μm,respectively.The thickness of the muscular layer in the anterior and posterior intestines were thicker than that in the midgut.The thickest values of the muscular layer in the anterior and posterior intestines are(339.76±22.56)μm and(251.59±26.94)μm,respectively.The thickest value of the muscular layer in the midgut is(199.91±25.16)μm(P＜0.05).AB-PAS staining showed the density of goblet cells in hindgut was significantly higher than foregut(P＜0.05).This study enriches the intestinal physiology and intestinal zoning of largemouth bass.
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