Construction and Immunogenicity Evaluation of Porcine deltacoronavirus Spike Protein Nanoparticles
To evaluate the immunogenicity of Porcine deltacoronaviurs(PDCoV)spike(S)proteinnanoparticles,the recombinant proteins S1-CTD-Spy-His and SpyCatcher-mi3 were firstly expressed by using pcDNA3.1(+)-S1-CTD-Spa-His and pET28a-SpyCatcher-mi3,After purification,SDS-PAGE identification showed that there were target bands at 55 ku and 37 ku.Utilizing the SpyTag-SpyCatcher protein ligase system,S1-CTD-Spycatcher-mi3 nanoparticles were obtained,and the diameter of 35～40 nm nanoparticles were observed under electron microscopy.The results of serum antibody assay showed that S1-CTD-SpyCatcher-mi3 nanoparticles induced a strong humoral immune response in mice,and the specific IgG antibody levels of the S1-CTD-SpyCatcher-mi3 group were significantly higher than those of the S1-CTD group(P＜0.05),and the differences between the S1-CTD-SpyCatcher-mi3 group and the S1-CTD group compared with the PBS control group were both highly significant(P＜0.01).This study shows S1-CTD-SpyCatcher-mi3 nanoparticles have good immunogenicity and can induce immune protection in mice,which lays a foundation for the development of novel Porcine deltacoronavirus infection vaccine.
国家科技期刊平台
NSTL
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