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蜂蜜外泌体的提取与鉴定

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外泌体是细胞或细菌分泌的纳米级小细胞外囊泡,在生理调整与病理发展当中发挥独特作用,而蜂蜜长久以来广泛被民间应用于人体医疗当中,目前已有足够的研究探索蜂蜜广谱的医学作用背后的各类原理,但对于蜂蜜中外泌体在蜂蜜医学实践当中的影响尚缺乏深一步的研究。为探究蜂蜜中外泌体的存在与其医学潜能,该研究分别对新鲜与陈放1年的蜂蜜样本采用超高速离心法的方式提取到其中的蜂蜜外泌体,并使用透射电镜与纳米颗粒跟踪仪对提取样本进行各类表征分析,发现了蜂蜜中外泌体大量存在,并能够在蜂蜜中长期室温保存。该研究证明了蜂蜜外泌体的开发前景,对蜂蜜外泌体的进一步开发具有指导作用。
Extraction and Identification of Honey Exosomes
Extracellular vesicles are nanoscale small extracellular vesicles secreted by cells or bacteria,which play a unique role in physiological regulation and pathological development.Honey has long been widely used in human medical care.At present,there are enough studies to explore the various principles behind the broad-spectrum medical effects of honey,but there is still a lack of further research on the influence of honey exosomes in honey medical practice.In order to explore the existence and medical potential of honey exosomes,this study extracted honey exosomes from fresh and aged honey samples for one year using high-speed centrifugation and conducted various characterization analyses.It was found that a large amount of honey exosomes exist and can be stored at room temperature for a long time in honey,proving the development prospects of honey exosomes and providing guidance for the further development of honey exosomes.

honeyexosomeextractidentification

王笑语、王承丽、吴攀、陈红建、岳万福

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浙江农林大学,浙江 杭州 311300

杭州临安红建蜜蜂养殖场,浙江 杭州 311300

蜂蜜 外泌体 提取 鉴定

遂昌县县校合作项目

2022HZ18

2024

现代畜牧科技
黑龙江省畜牧研究所

现代畜牧科技

影响因子:0.066
ISSN:2095-9737
年,卷(期):2024.(10)