现代畜牧科技2024,Issue(10) :49-52.DOI:10.19369/j.cnki.2095-9737.2024.10.015

复合诱变筛选提高花生粕中AFB1降解率的菌株

The Screening of Strains with Higher Degradation Rate of AFB1 in Peanut Meal by Compound Mutagenesis

宋静静 王克芬 张辉 张强 张法玲 滕来宾 赵遵明
现代畜牧科技2024,Issue(10) :49-52.DOI:10.19369/j.cnki.2095-9737.2024.10.015

复合诱变筛选提高花生粕中AFB1降解率的菌株

The Screening of Strains with Higher Degradation Rate of AFB1 in Peanut Meal by Compound Mutagenesis

宋静静 1王克芬 1张辉 1张强 1张法玲 1滕来宾 1赵遵明2
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作者信息

  • 1. 山东隆科特酶制剂有限公司,山东省酶制剂发酵技术重点实验室,山东 临沂 276400
  • 2. 山东省沂水县动物疫病预防控制中心,山东 临沂 276400
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摘要

选用产葡萄糖氧化酶(GOD)的黑曲霉菌株H-01为诱变出发菌株,经ARTP-NTG复合诱变后,筛选出1株酶活力提高46%的突变株HAN-40.在酶解条件:温度40℃,料液质量体积比1∶5,GOD添加量5 U/g,酶解时间24 h,突变株HAN-40较原始菌株所产GOD对花生粕中AFB1降解率提高了35%,达到92%.

Abstract

Aspergillus niger strain H-01,which produces glucose oxidase(GOD),was selected as the mutation strain.After the combined mutation of ARTP-NTG,a mutant strain named HAN-40 was selected.The enzyme activity of GOD produced by HAN-40 increased by 46%,which was higher than that of the original strain.Under the enzymolysis conditions:40℃,the mass volume ratio of solid to liquid 1∶5,GOD dosage 5 U/g,the enzymolysis time 24 h,the degradation rate of AFB1 in peanut meal of GOD produced by mutant HAN-40 increased by 35%compared with the original strain.And the degradation rate of AFB1 in peanut meal reached to 92%.

关键词

花生粕/黄曲霉毒素/葡萄糖氧化酶/诱变

Key words

peanut meal/aflatoxin/glucose oxidase/mutagenesis

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基金项目

山东省重点研发计划(2022CXGC020801)

出版年

2024
现代畜牧科技
黑龙江省畜牧研究所

现代畜牧科技

影响因子:0.066
ISSN:2095-9737
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