The Screening of Strains with Higher Degradation Rate of AFB1 in Peanut Meal by Compound Mutagenesis
Aspergillus niger strain H-01,which produces glucose oxidase(GOD),was selected as the mutation strain.After the combined mutation of ARTP-NTG,a mutant strain named HAN-40 was selected.The enzyme activity of GOD produced by HAN-40 increased by 46%,which was higher than that of the original strain.Under the enzymolysis conditions:40℃,the mass volume ratio of solid to liquid 1∶5,GOD dosage 5 U/g,the enzymolysis time 24 h,the degradation rate of AFB1 in peanut meal of GOD produced by mutant HAN-40 increased by 35%compared with the original strain.And the degradation rate of AFB1 in peanut meal reached to 92%.
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