A Report on the Diagnosis and Treatment of Bovine Enterovirus
In order to determine the pathogens of the diseased calves in a cattle farm in Napo County,Baise City,Guangxi Province,this study collected fecal samples from diarrhoeic calves and performed a series of laboratory diagnostics such as Reverse Transcription-Polymerase Chain Reaction(RT-PCR)detection,product sequence amplification and homology analysis.The RT-PCR amplification results showed that the clinical sample was positive for bovine enterovirus;sequence analysis revealed that the detected virus belonged to enterovirus subtype E2,and no mixed infection with other viruses was observed.Based on the above diagnostic results,it could be confirmed that bovine enterovirus infection was present in this cattle farm.Therefore,a series of comprehensive preventive and control measures such as isolation,rehydration and disinfection were implemented for the sick calves,which effectively controlled the further spread and development of the epidemic.In this paper,the differential diagnosis of a case of bovine enterovirus disease in Baise,Guangxi and its prevention and control measures were summarized to provide reference for further study of bovine enterovirus in Guangxi.