Study on Physicochemical Properties of Bacteriocin of Lactic acid bacteria Resistant to the Main Pathogenic Bacteria of Porcine Endometritis and Genome Sequencing Analysis
The aim of this study is to screen a bacteriocin-producing Lactic acid bacteria with the best comprehensive antagonistic effect against the main pathogenic bacteria of porcine endometritis.Strain identification of vegetable-derived lactic acid bacteria(MR5)was performed by combining morphological characteristics with 16S rRNA sequencing analysis.The crude extract of MR5 bacteriocin was subjected to different temperature,acid-base and protease treatment tests to analyse its bacteriocin physicochemical properties.The biosafety of MR5 was assessed by whole genome sequencing analysis.The results showed that:MR5 was identified as Leuconostoc lactis.Its bacteriocin could still maintain high antibacterial activity under ultra-high temperature treatment at 121℃,and it could maintain good antibacterial activity under the environment of a wide range of pH(4～10).It was sensitive to trypsin and proteinase K.MR5 does not contain antibiotic resistance genes and has good biosafety.In conclusion,MR5 bacteriocin showed potent antibacterial activity and good stability against the target strain,and it is expected to be developed as a new drug for the treatment of porcine endometritis.
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