Determination of Clopidol and Dinitolmide Residues in Eggs based on Matrix Dispersion Solid-phase Extraction and High Performance Liquid Chromatography
Determination of Clopidol and Dinitolmide Residues in Eggs based on Matrix Dispersion Solid-phase Extraction and High Performance Liquid Chromatography
To establish a rapid,efficient,and cost-effective method for the simultaneous determination of clopidol and dinitolmide residues in eggs based on matrix solid-phase dispersion extraction and high performance liquid chromatography.Egg samples were extracted with a methanol-acetonitrile mixture,purified using a mixture of 200 mg octadecylsilane bonded phase and 50 mg primary secondary amine for dispersion solid-phase extraction,separated using a C18 column,and eluted with a gradient of water-acetonitrile as the mobile phase.Quantitative and qualitative analysis were conducted using an external standard method.Results show that the clopidol and dinitolmide exhibited good linear relationships in the range of 0.025～50.0 μg/mL(R2＞0.999).The limits of detection were 0.025 mg/kg and 0.050 mg/kg,while the limits of quantitation were 0.075 mg/kg and 0.100 mg/kg for clopidol and dinitolmide In the spiked recovery tests at levels of 0.1,2.5 mg/kg,and 5.0 mg/kg,the recovery rates for clopidol ranged from 87.70%～101.78%,with relative standard deviations ranging from 0.66%～5.41%.For dinitolmide,the recovery rates ranged from 84.99%～97.11%with relative standard deviations ranging from 0.95%～5.34%.This method is simple,with good purification effects and high recovery rates,making it suitable for the simultaneous quantitative detection of clopidol and dinitolmide residues in eggs.
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