In order to fully exploit local specialty wool sheep breeds and promote the improvement of fine wool sheep breeds,this study utilized Gansu Alpine Merino Sheep as the maternal line and introduced Australian Merino Sheep as the paternal line.Superovulation and intra-abdominal endoscopic artificial insemination were employed to produce high-quality embryos,which were then transplanted into Tibetan sheep receptors.Excellent Gansu Alpine Merino ewes at 5～6-year old were selected as donors,with Tibetan sheep serving as recipients.A decreasing dose of FSH was administered for superovulation.After synchronized estrus,superovulation,and artificial insemination,embryos were surgically recovered from the uterine horns of the donors and subsequently transplanted into the recipients.The results indicated that 25 donors yielded a total of 130 embryos,with 66 available embryos obtained.The average number of embryos per donor was 5.2,while the average number of available embryos per donor was 2.64.After transplanting 66 embryos into 66 receptors,34 receptors became pregnant at a pregnancy rate of 51.52%.This experiment holds significant reference value in exploring suitable embryo transplantation technology tailored to local characteristics and leveraging regional resources to facilitate substantial improvements in the Gansu Alpine Merino Sheep breed.
Australian Merino sheepChinese Merino sheepsuperovulationembryo transfer
万方数据
维普
