猪链球菌肽链内切酶O(PepOSS)引起细胞免疫反应的研究
Study on Cellular Immune Response Induced by Endopeptidase O(PepOSS)of Streptococcus suis
冯于童 1李宇航 1胡金旺 1董芳婷 1闫一博 2张越1
作者信息
- 1. 河南农业大学动物医学院,河南 郑州 450046
- 2. 河南牧业经济学院动物医药学院,河南 郑州 450046
- 折叠
摘要
猪链球菌是全球生猪养殖业的重要病原菌,也是一种重要的人兽共患病病原.该研究在猪链球菌中鉴定了一个新的金属肽链内切酶PepOSS.利用在线工具对PepOSS氨基酸序列进行生物信息学分析,进行PepOSS蛋白表达及其兔源多克隆抗体的制备;并探究PepOSS蛋白对3D4/21细胞的免疫反应.结果显示,PepOSS属于肽酶M13家族;制备的多克隆抗体能与PepOSS蛋白产生特异性反应;PepOSS蛋白可引起3D4/21细胞天然免疫应答反应,经过PepOSS蛋白刺激后,IL-6、TLR4、CXCL10等细胞因子的mRNA表达量显著增加,且能增强细胞的吞噬作用;并证实pepOSS基因的表达受二元信号转导系统VraSRSS的调控.该研究为后续PepOSS相关研究提供了良好的理论依据.
Abstract
Streptococcus suis(SS)is a globally significant pathogen in pig breeding and also poses a considerable zoonotic risk.In this study,a novel metal endopeptidase PepOSS was identified in SS.The amino acid sequence of PepOSS was analyzed by bioinformatics using online tools,and PepOSS protein expression and rabbit polyclonal antibody preparation were performed.The immune response of PepOSS protein to 3D4/21 cells was investigated.The results revealed that PepOSS belonged to the peptidase M13 family.The prepared polyclonal antibody exhibited reactivity towards PepOSS protein.Moreover,PepOSS protein induced a natural immune response in 3D4/21 cells,leading to significant increases in mRNA expressions of IL-6,TLR4,CXCL10,and other cytokines as well as enhanced cell phagocytosis.Additionally,it was found that the expression of pepOSS gene is regulated by the two component signaling systems VraSRSS.This study provides a solid theoretical foundation for future research related to PepOSS.
关键词
链球菌/PepOSS蛋白/细胞因子/免疫应答Key words
Streptococcus/PepOSS/cytokine/immune response引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据