Isolation and Identification of Bovine Escherichia coli and Detection of Major Virulence Factors
This study aims to monitor the prevalence of Escherichia coli in dairy cows with mastitis and the virulence genes carried by dairy cows in a large-scale dairy farm.Diseased milk samples were collected from the field,and the pathogen was isolated and cultured first,and then PCR identification and homology comparison were performed.The results showed that the pathogenic bacteria were isolated from the milk samples of dairy cows,and the colony morphology of the isolated strains in McConkey medium was consistent with that of Escherichia coli.Amplification of 16S rDNA was consistent with expectations,resulting in a band of interest of approximately 544 bp in size.The results of the study confirmed that the pathogen causing mastitis in dairy cows was Escherichia coli,which provided a scientific basis for the clinical treatment and prevention and control of the disease.
Escherichia colimastitisisolation and identification of pathogenvirulent factor
国家科技期刊平台
NSTL
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