牛源大肠埃希菌的分离鉴定及主要毒力因子检测
Isolation and Identification of Bovine Escherichia coli and Detection of Major Virulence Factors
朱其保 1陈水云 1贾中辉 1郑志豪 1陈雪龙1
作者信息
- 1. 安徽科技学院,安徽 凤阳 233100
- 折叠
摘要
为监测某规模化奶牛场奶牛乳房炎大肠埃希菌的流行情况及携带的毒力基因,对该场进行患病乳样采集,先进行病原的分离培养,再进行PCR鉴定和同源性比较.结果显示,从奶牛的乳样中分离得到病细菌,分离菌株在麦康凯培养基菌落形态均与大肠埃希菌一致;扩增16S rDNA结果与预期相符,得到大小约为544 bp的目的条带.研究结果证实,引起奶牛乳房炎的病原为大肠埃希菌,为该病的临床治疗和防控提供科学依据.
Abstract
This study aims to monitor the prevalence of Escherichia coli in dairy cows with mastitis and the virulence genes carried by dairy cows in a large-scale dairy farm.Diseased milk samples were collected from the field,and the pathogen was isolated and cultured first,and then PCR identification and homology comparison were performed.The results showed that the pathogenic bacteria were isolated from the milk samples of dairy cows,and the colony morphology of the isolated strains in McConkey medium was consistent with that of Escherichia coli.Amplification of 16S rDNA was consistent with expectations,resulting in a band of interest of approximately 544 bp in size.The results of the study confirmed that the pathogen causing mastitis in dairy cows was Escherichia coli,which provided a scientific basis for the clinical treatment and prevention and control of the disease.
关键词
大肠埃希菌/乳房炎/病原分离鉴定/毒力因子Key words
Escherichia coli/mastitis/isolation and identification of pathogen/virulent factor引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据